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[Culture and characteristics of human dental papilla cells in vitro].
Xie, Jia-Min; Tian, Wei-Dong; Tang, Wei; Chen, Xi-Zhe; Zheng, Xiao-Hui; Wang, Tao.
Afiliação
  • Xie JM; Key Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 23(3): 187-90, 2005 Jun.
Article em Zh | MEDLINE | ID: mdl-16078451
ABSTRACT

OBJECTIVE:

To culture human dental papilla cells (HDPCs)and to study its cytobiological characters in vitro.

METHODS:

HDPCs were isolated and cultured with explant culture technique in vitro; Type I collagen, fibronection and laminin were detected in HDPCs and its secreted matrix with the immunocyto-chemical stain; HDPCs were incubated in mineralized promoting solution containing 10 mmol/L beta-glycerophosphate, 100 mg/L of ascorbic acid and 10 nmol/L dexamethasone supplemented with 10% FBS and the form of mineralized nodules was tested with Alizarin Red S stainning.

RESULTS:

Cultured HDPCs in vitro were well growing in DMEM/F12. Type I collagen, fibronection and laminin staining were all positive in both HDPCs and its secreted matrix, and laminin was stained with bunchiness in matrix. Mineralized nodules formed after cultured 27 days by Alizarin Red S stainning.

CONCLUSION:

HDPCs isolated and cultured are well growing in vitro, have a capability of synthesizing and secreting matrix and in mineralized promoting solution, are able to form mineralizer, so, HDPCs have a capacity of seed cell of tissue engineering regeneration tooth.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Engenharia Tecidual / Papila Dentária Limite: Humans Idioma: Zh Ano de publicação: 2005 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Engenharia Tecidual / Papila Dentária Limite: Humans Idioma: Zh Ano de publicação: 2005 Tipo de documento: Article