Site-specific transgenesis by Cre-mediated recombination in Drosophila.
Nat Methods
; 2(8): 583-5, 2005 Aug.
Article
em En
| MEDLINE
| ID: mdl-16094382
ABSTRACT
Transposons such as P elements are routinely used to stably transfer exogenous DNA (transgenes) into the Drosophila genome. Transgene insertion events, however, are essentially random and are subject to 'position effects' from nearby endogenous regulatory elements. Here we describe a microinjection-based system that uses Cre-mediated recombination to insert transgenes into precise genomic 'landing sites'. The system is simple and efficient, and will permit precise comparisons between multiple transgenic constructs.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Proteínas Virais
/
Técnicas de Transferência de Genes
/
Marcação de Genes
/
Transgenes
/
Integrases
/
Proteínas de Drosophila
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article