Your browser doesn't support javascript.
loading
Distinctive nuclear organisation of centromeres and regions involved in pluripotency in human embryonic stem cells.
Wiblin, Anne E; Cui, Wei; Clark, A John; Bickmore, Wendy A.
Afiliação
  • Wiblin AE; MRC Human Genetics Unit, Crewe Road, Edinburgh, EH4 2XU, UK.
J Cell Sci ; 118(Pt 17): 3861-8, 2005 Sep 01.
Article em En | MEDLINE | ID: mdl-16105879
ABSTRACT
Nuclear organisation is thought to be important in regulating gene expression. Here we investigate whether human embryonic stem cells (hES) have a particular nuclear organisation, which could be important for maintaining their pluripotent state. We found that whereas the nuclei of hES cells have a general gene-density-related radial organisation of chromosomes, as is seen in differentiated cells, there are also distinctive localisations for chromosome regions and gene loci with a role in pluripotency. Chromosome 12p, a region of the human genome that contains clustered pluripotency genes including NANOG, has a more central nuclear localisation in ES cells than in differentiated cells. On chromosome 6p we find no overall change in nuclear chromosome position, but instead we detect a relocalisation of the OCT4 locus, to a position outside its chromosome territory. There is also a smaller proportion of centromeres located close to the nuclear periphery in hES cells compared to differentiated cells. We conclude that hES cell nuclei have a distinct nuclear architecture, especially at loci involved in maintaining pluripotency. Understanding this level of hES cell biology provides a framework within which other large-scale chromatin changes that may accompany differentiation can be considered.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Núcleo Celular / Centrômero / Células-Tronco Pluripotentes Limite: Animals / Humans Idioma: En Ano de publicação: 2005 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Núcleo Celular / Centrômero / Células-Tronco Pluripotentes Limite: Animals / Humans Idioma: En Ano de publicação: 2005 Tipo de documento: Article