A restriction enzyme-based assay to distinguish between avian hemosporidians.
J Parasitol
; 91(3): 683-5, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-16108566
ABSTRACT
We describe a reliable and relatively inexpensive method for detecting and differentiating between the commonly studied avian blood parasite genera Haemoproteus, Plasmodium, and Leucocytozoon. The assay takes advantage of a Haemoproteus-specific restriction site identified by sequencing full mitochondrial genomes from two Haemoproteus and three Plasmodium lineages and an adjacent, genus-specific restriction site identified in Leucocytozoon spp. The assay was sensitive to simulated parasitemias of approximately 8 x 10(-6) per erythrocyte and was 100% accurate in differentiating between parasite genera isolated from a broad geographical and taxonomic sampling of infected hosts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Protozoárias em Animais
/
Doenças das Aves
/
DNA Mitocondrial
/
Mapeamento por Restrição
/
Haemosporida
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article