Determination of dihydroxynaphthalenes in human urine by gas chromatography-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci
; 826(1-2): 206-13, 2005 Nov 05.
Article
em En
| MEDLINE
| ID: mdl-16169295
ABSTRACT
A gas chromatography-mass spectrometry (GC-MS) method was developed for measuring 1,2-dihydroxynaphthalene (1,2-DHN) and 1,4-dihydroxynaphthalene (1,4-DHN) in urine. The method involves enzymatic digestion of urinary conjugates to release the DHNs which were then analyzed as trimethylsilyl derivatives by GC-MS. For 1,2-DHN and 1,4-DHN, respectively, the assay limits of detection were 0.21 and 0.15 microg/l, the assay limits of quantitation were 0.69 and 0.44 microg/l, and the coefficients of variation were 14.7 and 10.9%. This method was successfully applied to determine urinary levels of 1,2-DHN and 1,4-DHN in coke workers (14 top workers and 13 side-bottom workers) and 21 matching control workers from the steel industry of northern China. The geometric mean (GM) levels of 1,2-DHN were approximately 100 and 30 times higher than those of 1,4-DHN in exposed and control subjects, respectively. The GM levels 1,2-DHN and 1,4-DHN were significantly higher for coke workers (1,2-DHN top workers--552 microg/l, side-bottom workers--260 microg/l; 1,4-DHN top workers--3.42 microg/l, side-bottom workers--3.56 microg/l) than for controls (1,2-DHN 38.8 microg/l; 1,4-DHN 1.21 microg/l) (p
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidroquinonas
/
Cromatografia Gasosa-Espectrometria de Massas
/
Naftóis
Limite:
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article