Stability of immobilized soybean lipoxygenase in selected organic solvent media.
Appl Biochem Biotechnol
; 127(1): 29-42, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-16186621
ABSTRACT
The immobilization and biocatalysis of commercially purified soybean lipoxygenase (LOX) type I-B (EC 1.13.11.12) were investigated in organic solvent media. The results showed that the highest immobilization efficiencies of LOX, 30.6 and 29.3%, were obtained with DEAE-cellulose and modified Eupergit C250L supports, respectively. The biocatalysis of free and immobilized (Eupergit C250L/EDA) LOXs was investigated in different mixtures of hexane and a selected cosolvent (955 [v/v]). The results showed a 1.5 and a 1.6 increase in the activity of free and immobilized LOXs, respectively, using a mixture of hexane and 1,4-dioxane compared with that in hexane alone; however, cosolvents, including 2-octanone, 2-heptanone, 2-butanone, and cyclohexanone, displayed an inhibitory effect on LOX activity. In the mixture of 1,4-dioxane and hexane, LOX activity was dependent on the cosolvent concentration, which was increased with 1,4-dioxane up to 5% (v/v). The threshold 1,4-dioxane concentration (C50) and the incubation period (T50) at which 50% of the maximal enzyme activity was obtained for the free and immobilized LOXs were 6.7 and 8.9% (v/v) and 9.1 and 17.0 min, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Solventes
/
Glycine max
/
Lipoxigenase
/
Enzimas Imobilizadas
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article