Synthesis and biological activity of new iodoacetamide derivatives on mutants of squalene-hopene cyclase.
Lipids
; 40(7): 729-35, 2005 Jul.
Article
em En
| MEDLINE
| ID: mdl-16196424
ABSTRACT
New iodoacetamide derivatives, containing a dodecyl or a squalenyl moiety, were synthesized. The effect of these new thiol-reacting molecules was studied on two mutants of Alicyclobacillus acidocaldarius squalene-hopene cyclase constructed especially for this purpose. In the quintuple mutant, all five cysteine residues of the enzyme are substituted with serine; in the sextuple mutant, this quintuple substitution is accompanied by the substitution of aspartate D376, located at the enzyme's active site, with a cysteine. N-Dodecyliodoacetamide had little activity toward either mutant, whereas N-squalenyliodoacetamide showed a stronger effect on the sextuple than on the quintuple mutant, as expected.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transferases Intramoleculares
/
Iodoacetamida
/
Mutação
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article