Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells.
World J Gastroenterol
; 11(38): 6053-5, 2005 Oct 14.
Article
em En
| MEDLINE
| ID: mdl-16273624
ABSTRACT
AIM:
To study the function of alpha-fetoprotein (AFP) in SMMC-7721 hepatoma cells.METHODS:
A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescence activated cell sorter (FACS).RESULTS:
The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis.CONCLUSION:
Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alfa-Fetoproteínas
Limite:
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article