Estrogen receptors in the chick oviduct.
Endocrinology
; 96(1): 199-205, 1975 Jan.
Article
em En
| MEDLINE
| ID: mdl-162878
ABSTRACT
An estradiol binding component has been identified in the cytoplasmic fraction of the immature chick oviduct. The method used to resolve this receptor differed from the standard sucrose gradient centrifugation approach in that tritiated hormone was present throughout the sucrose gradient. This modification was necessary to preserve the hormone complex during centrifugation. Under these conditions, an similar to 8 S binding component was demonstrated which underwent dissociation to a similar to 5 S component in high ionic strength medium. Binding specificity determinations revealed that this receptor preferentially bound estrogens. Quantitative binding analysis showed that a limited class of binding sites was present with a dissociation constant (K-d) for estradiol of similar to 8.6 times 10-10M. These properties indicate that this binding component may function as a biologic receptor for estrogens in the oviduct.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oviductos
/
Receptores de Superfície Celular
/
Estradiol
Limite:
Animals
Idioma:
En
Ano de publicação:
1975
Tipo de documento:
Article