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Head and neck squamous cell carcinoma spheroid- and monocyte spheroid-stimulated IL-6 and monocyte chemotactic protein-1 secretion are related to TNM stage, inflammatory state and tumor macrophage density.
Kross, Kenneth W; Heimdal, John-Helge; Olsnes, Carla; Olofsson, Jan; Aarstad, Hans Jørgen.
Afiliação
  • Kross KW; Department of Otolaryngology/Head and Neck Surgery, Haukeland University Hospital and Broegelmann Research Laboratory, University of Bergen, Bergen, Norway.
Acta Otolaryngol ; 125(10): 1097-104, 2005 Oct.
Article em En | MEDLINE | ID: mdl-16298793
ABSTRACT
Monocyte fragment (F)-spheroid-stimulated and F-spheroid IL-6 and monocyte chemotactic protein (MCP)-1 secretion are related to inflammatory state, macrophage density and the TNM stage of patients with head and neck squamous cell carcinoma (HNSCC). Fragment (F)-spheroids from HNSCC patients in vitro secrete and stimulate autologous monocytes to secrete IL-6 and MCP-1. The aim of this investigation was to study this cytokine secretion in relation to other cytokines, spheroid composition and host factors.In series I (n=14) the densities of epithelial cells, fibroblasts and macrophages were determined in sections from F-spheroids and donor tissue. In series II (n=17) the TNM stage, donor inflammatory state, macrophage density and the secretion of F-spheroid- and monocyte F-spheroid-stimulated IL-6, MCP-1 and tumor necrosis factor (TNF)-alpha were determined. Epithelial cells were partly replaced by interstitial tissue during spheroid formation. Malignant (M) F-spheroids secreted more MCP-1 than benign (B) F-spheroids. No F-spheroid secreted measurable amounts of TNF-alpha. Monocytes secreted more IL-6 when co-cultured with MF- compared to BF-spheroids. Monocyte IL-6 MF- and MCP-1 MB-spheroid-stimulated secretion correlated with macrophage density. In addition, there was an association between MF- and BF-spheroid-stimulated monocyte cytokine secretion, as well as between BF- and MF-spheroid-stimulated MCP-1 secretion. An inverse relation was also noted between the erythrocyte sedimentation rate at monocyte harvest and the monocyte MCP-1 F-spheroid responses.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Monócitos / Interleucina-6 / Esferoides Celulares / Quimiocina CCL2 / Neoplasias de Cabeça e Pescoço Limite: Humans Idioma: En Ano de publicação: 2005 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Monócitos / Interleucina-6 / Esferoides Celulares / Quimiocina CCL2 / Neoplasias de Cabeça e Pescoço Limite: Humans Idioma: En Ano de publicação: 2005 Tipo de documento: Article