Understanding the response of dendritic cells to activation by streptococcal preparation OK-432.
Anticancer Res
; 25(6B): 4231-8, 2005.
Article
em En
| MEDLINE
| ID: mdl-16309221
ABSTRACT
BACKGROUND:
The streptococcal preparation OK-432 induces maturation of T cells and dendritic cells (DCs). However, the mechanisms by which OK-432 induces DC maturation are not well understood. MATERIALS ANDMETHODS:
The effects of OK-432 and TNF-alpha on peripheral blood mononuclear cells (PBMCs) were compared. Antibody-based approaches were used to detect proteins characteristic of antigen-presenting cells and cytokines. Cytotoxicity was evaluated by measuring the release of LDH after incubation of effector and target cells. The TLR-4 levels were measured by real-time quantitative PCR.RESULTS:
Changes in cell surface marker levels were detected in both treatment groups but OK-432 had a greater effect on induction of Th-1-type cytokines. Furthermore, TLR-4 mRNA was up-regulated in cells from two out of five patients in response to OK-432, but not in response to TNF-alpha.CONCLUSION:
OK-432 has a more profound effect on human DCs than TNF-alpha and may act through multiple signaling pathways.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Picibanil
/
Células Dendríticas
Limite:
Adult
/
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article