Xenopus apyrase (xapy), a secreted nucleotidase that is expressed during early development.
Gene
; 367: 135-41, 2006 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-16314051
ABSTRACT
We have characterized a cDNA encoding a Xenopus laevis apyrase (XAPY) that is expressed during embryogenesis. XAPY is highly homologous to two recently described mammalian apyrases, human SCAN-1 and rat Ca2+-NDPase, and to a lesser extent the salivary apyrase of the blood-feeding arthropod Cimex lectularis. RT-PCR analysis shows that Xapy is expressed at all the developmental stages tested, from oocytes through to tadpoles. Xapy transcripts are widely distributed in the embryo, but from late neurulae through to late tailbud stages they are highly enriched in the cement gland, an adhesive organ in the epidermis of the head. When expressed in HEK 293 cells, XAPY is largely retained in the endoplasmic reticulum, although some is also secreted. XAPY conditioned media hydrolyses UDP and UTP, confirming that it is a functional apyrase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apirase
/
Xenopus
/
Regulação da Expressão Gênica no Desenvolvimento
/
Nucleotidases
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article