Histopathological and behavioral improvement of murine mucopolysaccharidosis type VII by intracerebral transplantation of neural stem cells.
Mol Ther
; 13(3): 548-55, 2006 Mar.
Article
em En
| MEDLINE
| ID: mdl-16316785
ABSTRACT
The therapeutic efficacy of neural stem cell transplantation for central nervous system (CNS) lesions in lysosomal storage disorders was explored using a murine model of mucopolysaccharidosis type VII (MPS VII). We used fetal neural stem cells derived from embryonic mouse striata and expanded in vitro by neurosphere formation as the source of graft materials. We transplanted neurospheres into the lateral ventricles of newborn MPS VII mice and found that donor cells migrated far beyond the site of injection within 24 h, and some of them could reach the olfactory bulb. A quantitative measurement indicated that the GUSB activity in the brain was 12.5 to 42.3% and 5.5 to 6.3% of normal activity at 24 h and 3 weeks after transplantation. In addition, histological analysis revealed a widespread decrease in lysosomal storage in the recipient's hippocampus, cortex, and ependyma. A functional assessment with novel-object recognition tests confirmed improvements in behavioral patterns. These results suggest that intracerebral transplantation of neural stem cells is feasible for treatment of CNS lesions associated with lysosomal storage disorders.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Telencéfalo
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Terapia Genética
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Mucopolissacaridose VII
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Transplante de Células-Tronco
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Glucuronidase
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article