Tumor necrosis factor-alpha enhances Haemophilus somnus lipooligosaccharide-induced apoptosis of bovine endothelial cells.
Vet Immunol Immunopathol
; 110(3-4): 303-9, 2006 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-16325265
ABSTRACT
Haemophilus somnus lipooligosaccharide (LOS)-induced apoptosis of bovine pulmonary artery endothelial cells has been shown previously to be dependent on caspase-8 activation. Activation of caspase-8 can occur via a death receptor-dependent mechanism (e.g., TNF-alpha binding to TNF-alpha receptor 1 (TNF-R1)). In this study, we tested the hypothesis that TNF-alpha can enhance LOS-induced apoptosis of bovine endothelial cells. Addition of exogenous recombinant human TNF-alpha alone failed to cause apoptosis, or enhance LOS-induced apoptosis, of bovine endothelial cells. However, blocking de novo protein synthesis by addition of cycloheximide significantly enhanced apoptosis of bovine endothelial cells by TNF-alpha, LOS or TNF-alpha and LOS in combination. Conversely, addition of soluble recombinant human (sTNF-R1) diminished LOS-induced apoptosis. Overall, these data suggest that LOS-mediated apoptosis may be due, in part, to activation of a TNR-R1-dependent death pathway.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
Fator de Necrose Tumoral alfa
/
Apoptose
/
Células Endoteliais
/
Haemophilus somnus
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article