Quantitative analysis of PPARdelta mRNA by real-time RT-PCR in 86 rectal cancer tissues.

ABSTRACT

AIM: The purpose of this study is to clarify the expression change of PPARdelta gene in human colorectal cancer tissues. METHODS: Applying real-time RT-PCR, we quantified PPARdelta mRNA in a series of 86 tissues from excised primary rectal cancers. In each case, accompanying normal mucosa was collected for comparison. RESULTS: Among the 86 rectal cancer tissues, 48 cases showed PPARdelta overexpression 39 tumours gave an expression level 1.5-5 times, five tumours 10-20 times, and four tumours more than 20 times relative to normal mucosa. However, the general level of PPARdelta mRNA in rectal cancer tissues is not statistically different from normal mucosa. CONCLUSIONS: The expression of PPARdelta gene in rectal cancers is not statistically different from normal mucosa.