A mutation inactivating the methyltransferase gene in avirulent Madrid E strain of Rickettsia prowazekii reverted to wild type in the virulent revertant strain Evir.
Vaccine
; 24(13): 2317-23, 2006 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-16364512
ABSTRACT
Rickettsia prowazekii Madrid E (E) strain is an effective vaccine, but can revert to virulent status when passaged in animals. The aim of this study is to identify the reverse mutation that may determine the virulence of R. prowazekii by comparing the genetic structures of E strain and its virulent revertant Evir strain. We determined that the gene (Rp028/Rp027) encoding the methyltransferase was mutated by frameshift in avirulent E strain but not in virulent revertant Evir strain and wild type virulent Breinl strain. We conclude that the mutation in the E strain gene reverts to wild type in the virulent revertant Evir strain. Whether the mutation plays an essential role in the attenuation of E strain needs to be further investigated.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rickettsia prowazekii
/
Metiltransferases
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article