Your browser doesn't support javascript.
loading
UL54-null pseudorabies virus is attenuated in mice but productively infects cells in culture.
Schwartz, Jennifer A; Brittle, Elizabeth E; Reynolds, Ashley E; Enquist, Lynn W; Silverstein, Saul J.
Afiliação
  • Schwartz JA; Department of Microbiology, Columbia University, 701 W. 168th St., New York, NY 10032, USA.
J Virol ; 80(2): 769-84, 2006 Jan.
Article em En | MEDLINE | ID: mdl-16378979
The pseudorabies virus (PRV) UL54 homologs are important multifunctional proteins with roles in shutoff of host protein synthesis, transactivation of virus and cellular genes, and regulation of splicing and translation. Here we describe the first genetic characterization of UL54. We constructed UL54 null mutations in a PRV bacterial artificial chromosome using sugar suicide and lambdaRed allele exchange systems. Surprisingly, UL54 is dispensable for growth in tissue culture but exhibits a small-plaque phenotype that can be complemented in trans by both the herpes simplex virus type 1 ICP27 and varicella-zoster virus open reading frame 4 proteins. Deletion of UL54 in the virus vJSdelta54 had no effect on the ability of the virus to shut off host cell protein synthesis but did affect virus gene expression. The glycoprotein gC accumulated to lower levels in cells infected with vJSdelta54 compared to those infected with wild-type virus, while gK levels were undetectable. Other late gene products, gB, gE, and Us9, accumulated to higher levels than those seen in cells infected with wild-type virus in a multiplicity-dependent manner. DNA replication is also reduced in cells infected with vJSdelta54. UL54 appears to regulate UL53 and UL52 at the transcriptional level as their respective RNAs are decreased in cells infected with vJSdelta54. Interestingly, vJSdelta54 is highly attenuated in a mouse model of PRV infection. Animals infected with vJSdelta54 survive twice as long as animals infected with wild-type virus, and this results in delayed accumulation of virus-specific antigens in skin, dorsal root ganglia, and spinal cord tissues.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudorraiva / Herpesvirus Suídeo 1 Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2006 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudorraiva / Herpesvirus Suídeo 1 Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2006 Tipo de documento: Article