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The first deltex null mutant indicates tissue-specific deltex-dependent Notch signaling in Drosophila.
Fuwa, Takashi J; Hori, Kazuya; Sasamura, Takeshi; Higgs, Jenny; Baron, Martin; Matsuno, Kenji.
Afiliação
  • Fuwa TJ; Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510 Noda, Chiba, Japan.
Mol Genet Genomics ; 275(3): 251-63, 2006 Mar.
Article em En | MEDLINE | ID: mdl-16395579
Notch (N) is a single-pass transmembrane receptor. The N signaling pathway is an evolutionarily conserved mechanism that controls various cell-specification processes. Drosophila Deltex (Dx), a RING-domain E3 ubiquitin ligase, binds to the N intracellular domain, promotes N's endocytic trafficking to late endosomes, and was proposed to activate Suppressor of Hairless [Su(H)]-independent N signaling. However, it has been difficult to evaluate the importance of dx, because no null mutant of a dx family gene has been available in any organism. Here, we report the first null mutant allele of Drosophila dx. We found that dx was involved only in the subsets of N signaling, but was not essential for it in any developmental context. A strong genetic interaction between dx and Su(H) suggested that dx might function in Su(H)-dependent N signaling. Our epistatic analyses suggested that dx functions downstream of the ligands and upstream of activated Su(H). We also uncovered a novel dx activity that suppressed N signaling downstream of N.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Proteínas de Drosophila / Receptores Notch / Proteínas de Membrana / Mutação Limite: Animals Idioma: En Ano de publicação: 2006 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Proteínas de Drosophila / Receptores Notch / Proteínas de Membrana / Mutação Limite: Animals Idioma: En Ano de publicação: 2006 Tipo de documento: Article