Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 62(Pt 3): 235-7, 2006 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-16511310
ABSTRACT
A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 A resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2(1)2(1)2(1). Crystallographic refinement and full amino-acid sequence determination are in progress.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sementes
/
Lectinas de Plantas
/
Fabaceae
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article