In vitro models for studying trophoblast transcellular transport.
Methods Mol Med
; 122: 225-39, 2006.
Article
em En
| MEDLINE
| ID: mdl-16511984
ABSTRACT
In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trofoblastos
/
Técnicas de Cultura de Células
/
Linhagem Celular Tumoral
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article