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Raman spectroscopy determines structural changes associated with gelation properties of fish proteins recovered at alkaline pH.
Thawornchinsombut, Supawan; Park, Jae W; Meng, Guangtao; Li-Chan, Eunice C Y.
Afiliação
  • Thawornchinsombut S; OSU Seafood Laboratory and Department of Food Science and Technology, Oregon State University, 2001 Marine Drive, Ste. 253, Astoria, Oregon 97103, USA.
J Agric Food Chem ; 54(6): 2178-87, 2006 Mar 22.
Article em En | MEDLINE | ID: mdl-16536593
Structural changes of alkali-treated rockfish protein isolate (AKPI) during frozen storage were elucidated using a Raman spectrometer and scanning electron microscope (SEM). The results were compared to conventional surimi (CS). No significant textural difference was noted between AKPI stored at pH 5.5 and 7.0. The strongest texture was found for AKPI frozen with cryoprotectants and CS, while the weakest texture was observed in AKPI frozen without cryoprotectants. SEM revealed the most discontinuity in gels of AKPI with no cryoprotectants and a more aggregated microstructure after storage at pH 5.5 than at neutral pH. Raman spectral analysis demonstrated refolding of AKPI by pH readjustment to 7.0, although the refolded structure was not identical to that before the pH shift. CS showed higher alpha-helix content (approximately 50%) than AKPI (approximately 20-30%). Frozen storage induced a decrease and an increase in the alpha-helix content of CS and AKPI samples, respectively. AKPIs were slightly less stable than CS during frozen storage.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Alimentos Marinhos / Proteínas de Peixes / Conservação de Alimentos / Géis Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2006 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Alimentos Marinhos / Proteínas de Peixes / Conservação de Alimentos / Géis Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2006 Tipo de documento: Article