Molecular characterization of beta-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A.
FEMS Microbiol Lett
; 257(2): 319-27, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16553870
ABSTRACT
The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA but not in blaB. Cluster analysis of blaA restriction profiles grouped the strains into three groups. The blaA gene of Y. enterocolitica biovar 1A showed a high degree of sequence homology to that of Y. enterocolitica 8081 (biovar 1B) and Y. enterocolitica Y-56 (biovar 4), whereas homology was low with class A beta-lactamase genes of other members of the family Enterobacteriaceae. The pI 8.7 of enzyme Bla-A of Y. enterocolitica biovar 1A was similar to that of biovars 2, 3 and 4. The enzyme Bla-B focused at 6.8 and 7.1, indicating that biovar 1A strains produced a 'B-like' enzyme. This is the first study to have investigated the genetic heterogeneity of the beta-lactamase genes of Y. enterocolitica.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Yersinia enterocolitica
/
Beta-Lactamases
Limite:
Animals
/
Humans
País/Região como assunto:
America do norte
/
Asia
/
Europa
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article