Expression of HMGA2 variants during oogenesis and early embryogenesis of Xenopus laevis.
Eur J Cell Biol
; 85(6): 519-28, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16584807
ABSTRACT
The high mobility group proteins A2 (HMGA2) have been implicated in the control of cell proliferation and differentiation, in particular during embryogenesis. Here, we used Xenopus laevis to analyze HMGA2 gene expression patterns during oogenesis and early embryogenesis. We found two functional XlHMGA2 isoforms, which we named XlHMGA2alpha and XlHMGA2beta. As revealed by RT-PCR, real-time PCR and whole-mount in situ hybridization both mRNAs are maternally produced and stored in eggs. Whole-mount in situ hybridizations revealed a conspicuous redistribution of the XlHMGA2 transcripts during early embryogenesis. Initially, during oogenesis and in eggs, the transcripts are uniformly distributed in the cytoplasm. With activation of the eggs the transcripts accumulate near the animal pole and remain in the juxtanuclear regions of animal pole blastomeres until midblastula transition. According to real-time PCR data, XlHMGA2alpha appears to be preferentially expressed during oogenesis and after midblastula transition, whereas XlHMGA2beta expression predominates after neurulation, suggesting an individual transcriptional regulation.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oogênese
/
Xenopus laevis
/
Regulação da Expressão Gênica no Desenvolvimento
/
Proteína HMGA2
/
Desenvolvimento Embrionário
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article