The relative stability of selected herpes simplex virus type 1 mRNAs.
Virus Res
; 20(2): 121-32, 1991 Jul.
Article
em En
| MEDLINE
| ID: mdl-1659058
ABSTRACT
The relative stability of herpes simplex virus type 1 mRNAs was investigated by examination of the decay rates of selected viral transcripts. The synthesis of mRNA was inhibited by the addition of dactinomycin to HSV-1 infected cells, and the abundance of individual transcripts was determined at subsequent times by RNA blot hybridization. For two immediate-early mRNAs, those encoding the 110 and 63 kilodalton immediate-early proteins, RNA synthesis was inhibited at 3 h post-infection and mRNA half-lives of 5-7 h were found. Examination at 5 h post-infection of the early mRNA encoding thymidine kinase as well as the late mRNA encoding glycoprotein H revealed half-lives of 8-11 h. In contrast, at 12 h post-infection, the late mRNAs encoding the glycoproteins C, E, as well as H were found to have half-lives of 14-29 h. These findings suggest that the relative stability of viral mRNA increases late in infection and is dependent upon the time after infection rather than being strictly a property of the mRNA itself.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
RNA Viral
/
Simplexvirus
Limite:
Animals
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article