Trichinella spiralis: histamine secretion induced by TSL-1 antigens from unsensitized mast cells.
Exp Parasitol
; 114(2): 67-76, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-16600218
ABSTRACT
Mast cells' hyperplasia and activation are prominent features in Trichinella spiralis infection. Recently, it was shown that TSL-1 antigens from T. spiralis muscle larvae induce IL-4 and TNF release by unsensitized, normal mast cells (MC) involving an Ig-independent mechanism. In this study, we characterized histamine secretion induced by TSL-1 antigens from normal, unsensitized rat peritoneal MC. Maximum histamine secretion (30+/-5.3% SEM, n=13) was achieved with 30 ng/mL TSL-1 antigens. However, TSL-1 did not induce an increase in beta-hexosaminidase release or NADPH oxidase activity by MC. Interestingly, histamine secretion by TSL-1 was completed at 10s, and was inhibited by both Bordetella pertussis toxin and neuraminidase V, characteristics similar to those involved in substance P-induced histamine secretion. However, in contrast to substance P, TSL-1 induced histamine secretion in the absence of detectable changes in intracellular Ca(2+). We are investigating the molecular pathways involved in MC activation by TSL-1.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trichinella spiralis
/
Liberação de Histamina
/
Mastócitos
/
Antígenos de Helmintos
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article