Evaluation of a novel Vi conjugate vaccine in a murine model of salmonellosis.
Vaccine
; 24(20): 4312-20, 2006 May 15.
Article
em En
| MEDLINE
| ID: mdl-16600446
ABSTRACT
Immunisation of BALB/c mice with a vaccine containing Vi polysaccharide conjugated to the Klebsiella pneumoniae outer membrane 40 kDa protein (rP40), in combination with Escherichia coli heat-labile toxin adjuvant (LT), elicited anti-Vi IgG antibodies after administration using different routes. Testing of the immune serum in opsonisation assays demonstrated the specific enhancement of Vi-positive bacterial uptake by cultured murine bone marrow derived macrophages. Intra-peritoneal challenge of mice immunised with the Vi-based vaccine elicited a degree of protection against virulent Vi+ Salmonella enterica serovar typhimurium (S. typhimurium). In contrast, Vi vaccination did not confer protection against oral challenge with virulent Vi-positive S. typhimurium or S. dublin.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
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Infecções por Salmonella
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Vacinas Bacterianas
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Modelos Animais de Doenças
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article