Protein identification and expression analysis using mass spectrometry.
Trends Microbiol
; 14(5): 229-35, 2006 May.
Article
em En
| MEDLINE
| ID: mdl-16603360
ABSTRACT
The identification and quantification of the proteins that a whole organism expresses under certain conditions is a main focus of high-throughput proteomics. Advanced proteomics approaches generate new biologically relevant data and potent hypotheses. A practical report of what proteome studies can and cannot accomplish in common laboratory settings is presented here. The review discusses the most popular tandem mass-spectrometry-based methods and focuses on how to produce reliable results. A step-by-step description of proteome experiments is given, including sample preparation, digestion, labeling, liquid chromatography, data processing, database searching and statistical analysis. The difficulties and bottlenecks of proteome analysis are addressed and the requirements for further improvements are discussed. Several diverse high-throughput proteomics-based studies of microorganisms are described.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Espectrometria de Massas por Ionização por Electrospray
/
Proteômica
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article