Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
Org Biomol Chem
; 4(8): 1458-60, 2006 Apr 21.
Article
em En
| MEDLINE
| ID: mdl-16604208
ABSTRACT
The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
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Coleções:
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Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Streptomyces
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Proteínas de Bactérias
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Desoxiadenosinas
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article