Development of affinity chromatography using a bioactive peptide as a ligand.
Bioorg Med Chem
; 14(15): 5093-8, 2006 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-16650997
ABSTRACT
By repeatedly introducing hydrophilic polyethylene glycol (PEG) spacer (2) onto affinity resin bearing a bioactive peptide (1/2 secretory leukocyte protease inhibitor, 1/2SLPI) as a ligand, the adsorption of nonspecific binding proteins was effectively reduced and the purification efficacy of elastase, which is one of the target molecules for 1/2SLPI, from a protein mixture was improved. Moreover, using this resin, we also successfully detected L-plastin, as an endogenous target molecule for SLPI, from HL-60 cell lysate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Proteínas
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article