[Study of the mutagenic properties of oligonucleotides containing and internucleotide pyrophosphate bond using a mutation system based on phage M13]. / Issledovanie mutagennykh svoistv oligonukleotidov, soderzhashchikh mezhnukleotidnoe pirofosfatnoe zveno, c pomoshch'iu mutatsionnoi sistemy na osnove faga M13.

ABSTRACT

Mutagenic properties of oligonucleotides with pyrophosphate internucleotide bond was studied. It was shown that the pyrophosphate bond in the oligo structure does not induce mutations but promotes a more efficient induction of marker deletions predetermined by the nucleotide sequence as compared to the native oligonucleotide. Marker deletion induction proceeds according to the repair mechanism as homozygotes dominate in the mutant generation.