[Effects of ERK5 MAPK signaling transduction pathway on the inhibition of genistein to breast cancer cells].
Wei Sheng Yan Jiu
; 35(2): 184-6, 2006 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-16758967
ABSTRACT
OBJECTIVE:
To study the role of ERK5 MAPK signaling transduction pathway in the inhibition of genistein to MDA-MB-231 breast cancer cells.METHODS:
The inhibitive effects of genistein on MDA-MB-231 cells were observed with the MTT assay. The cell apoptosis was detected by flow cytometry. The ERK5 MAPK, Bax and Caspase3 protein expressions were detected by using western blot method.RESULTS:
genistein inhibited the cell proliferation of MDA-MB-231 cells and induced apoptosis. The protein expression of ERK5 decreased and those of Bax and Caspase3 increased with the dose of genistein.CONCLUSION:
Genistein can affect the ERK5 MAPK signaling transduction pathway and induce the expressions of apoptosis related proteins to inhibit the proliferation of MDA-MB-231 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Transdução de Sinais
/
Apoptose
/
Genisteína
/
MAP Quinase Quinase 5
Limite:
Female
/
Humans
Idioma:
Zh
Ano de publicação:
2006
Tipo de documento:
Article