Apoptosis of human colon carcinoma HT-29 cells induced by ceramide.
World J Gastroenterol
; 12(22): 3581-4, 2006 Jun 14.
Article
em En
| MEDLINE
| ID: mdl-16773715
ABSTRACT
AIM:
To investigate the effect of exogenous ceramide-induced apoptosis on human colon carcinoma HT-29 cells.METHODS:
Light microscope, transmission electron microscope and fluorescence microscope were used to observe the morphology change of apoptosis in HT-29 cells. Agarose gel electrophoresis was performed to detect the DNA fragment. Mitochondrial function was detected by MTT assay. mRNA expression of Bcl-2 family gene members was determined by reverse transcription polymerase chain reaction (RT-PCR) assay.RESULTS:
After C2-ceramide treatment, typical characteristics of apoptosis, such as nuclear chromatin breakage, apoptotic body and DNA ladder, could be observed. After exposure to 50 micromol/L C2-ceramide for 12 and 24 h, cell apoptosis was 64.1% and 81.3% respectively, which had a time-and dose-effect relationship. Mitochondrial function started to decrease from 6 h after exposure to ceramide. Meanwhile, ceramide up-regulated or down-regulated the mRNA expression of Bcl-2 family gene members.CONCLUSION:
Ceramide induces apoptosis of human colon carcinoma HT-29 cells by affecting the expression of Bcl-2 family gene members and impacting the mitochondrial function.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esfingosina
/
Apoptose
/
Neoplasias do Colo
/
Inibidores Enzimáticos
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article