Expression and secretion of recombinant outer-surface protein A from the Lyme disease agent, Borrelia burgdorferi, in Nicotiana tabacum suspension cells.
Transgenic Res
; 15(3): 325-35, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16779648
ABSTRACT
The ospA gene of Borrelia burgdorferi codes for an outer membrane lipoprotein, which is a major antigen of the Lyme disease agent. Recombinant OspA vaccines tested so far were expressed in Escherichia coli. In this study, we investigated the expression of a soluble OspA protein in Nicotiana tabacum suspension cells and evaluated the secretion of OspA driven by either its own bacterial signal peptide or a plant signal peptide fused to the amino-terminal cysteine of the mature form. In both cases, the signal peptide was cleaved off and OspA secreted. During secretion, OspA was N-glycosylated. Addition of a C-terminal KDEL sequence led to retention of OspA in the endoplasmic reticulum.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nicotiana
/
Proteínas da Membrana Bacteriana Externa
/
Vacinas Bacterianas
/
Plantas Geneticamente Modificadas
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Regulação da Expressão Gênica de Plantas
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Borrelia burgdorferi
/
Lipoproteínas
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Antígenos de Superfície
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article