Development of restricted-access media supports and their application to the direct analysis of biological fluid samples via high-performance liquid chromatography.
Anal Bioanal Chem
; 384(7-8): 1462-9, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16800052
ABSTRACT
A quick overview of published methods for analyzing compounds in complex biological samples reveals that the most difficult step is the clean-up or extraction of a required compound from the matrix. The strategy required to analyze exogenous compounds in biological fluids depends greatly upon the nature of the compound and upon the biomatrix. Coupled-column separation using restricted-access media as the first dimension in order to exclude macromolecules and retain micromolecules has been successfully used for a number of biological fluids. This paper presents the history of the development of restricted-access media supports and of their application to the direct injection of biological fluid samples in high-performance liquid chromatography.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Química Analítica
/
Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article