Bleomycin-treated DNA is specifically cleaved only by endonuclease IV in E. coli.
Biochim Biophys Acta
; 1048(1): 19-23, 1990 Jan 30.
Article
em En
| MEDLINE
| ID: mdl-1688711
ABSTRACT
We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45 degrees C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV.
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Base de dados:
MEDLINE
Assunto principal:
Bleomicina
/
DNA
/
Proteínas de Escherichia coli
/
Endodesoxirribonucleases
/
Escherichia coli
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article