Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica.
Parasitology
; 133(Pt 6): 763-75, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-16938151
ABSTRACT
A cDNA encoding Fg14-3-3 protein 1 was cloned by immunoscreening of an adult-stage Fasciola gigantica cDNA library using a rabbit antiserum against tegumental antigens of the parasite. The protein has a deduced amino acid sequence of 252 residues and a calculated molecular weight of 28.7 kDa. It shows sequence identity values between 57.6 and 58.1% to the human 14-3-3 beta, zeta, theta, and eta proteins and is in a phylogenetic cluster with the 14-3-3 protein 1 of Schistosoma spp. Nucleic acid analyses indicate that the Fg14-3-3 protein 1 is encoded by a single copy gene and that this gene is expressed as a transcript of 1250 nucleotides. In adult and 4-week-old parasites the gene's transcriptional and translational products were localized in the gut epithelium, parenchyma, tegument cells, and in the reproductive organs. An antiserum against recombinant Fg14-3-3 protein 1 detected a slightly smaller 14-3-3 protein in the parasite's excretion/secretion material and showed cross-reactivity with 14-3-3 proteins in extracts of other trematodes and mouse. Antibodies against Fg14-3-3 protein were detected in the sera of rabbits as early as 2 weeks after infection with metacercariae of F. gigantica and the antibody titre increased continuously over a 10-week observation period.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas 14-3-3
/
Fasciola
/
Fasciolíase
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article