Melatonin prevents H2O2-induced activation of rat hepatic stellate cells.
J Pineal Res
; 41(3): 275-8, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-16948789
ABSTRACT
Considerable evidence shows therapeutic effects of melatonin on liver injury and the involvement of hepatic stellate cells (HSCs) in vivo. In the present studies, we investigate the protective effect of melatonin on H2O2-induced activation of HSCs in vitro. Compared with that in control HSCs, synthesis of collagen type I was increased in H2O2-treated cells. Melatonin pretreatment significantly inhibited the above effects of H2O2 in HSCs. CCAAT/enhancer-binding protein alpha (C/EBP-alpha), which could partially reverse the phenotype of activated HSCs, augmented in HSCs pretreated with melatonin. Moreover, secretion of the most important fibrotic cytokine transforming growth factor beta 1 (TGF-beta1) diminished in melatonin-pretreated HSCs. These results suggest that melatonin prevents H2O2-induced activation of HSCs and that the mechanism involves, at least in part, differential regulation of TGF-beta1 and C/EBP-alpha gene expression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxidantes
/
Peróxido de Hidrogênio
/
Fígado
/
Melatonina
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article