Humanization of yeast to produce complex terminally sialylated glycoproteins.
Science
; 313(5792): 1441-3, 2006 Sep 08.
Article
em En
| MEDLINE
| ID: mdl-16960007
ABSTRACT
Yeast is a widely used recombinant protein expression system. We expanded its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with fully complex terminally sialylated N-glycans. After the knockout of four genes to eliminate yeast-specific glycosylation, we introduced 14 heterologous genes, allowing us to replicate the sequential steps of human glycosylation. The reported cell lines produce complex glycoproteins with greater than 90% terminal sialylation. Finally, to demonstrate the utility of these yeast strains, functional recombinant erythropoietin was produced.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pichia
/
Sialoglicoproteínas
/
Engenharia de Proteínas
/
Eritropoetina
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article