Interactions between PII proteins and the nitrogenase regulatory enzymes DraT and DraG in Azospirillum brasilense.
FEBS Lett
; 580(22): 5232-6, 2006 Oct 02.
Article
em En
| MEDLINE
| ID: mdl-16963029
ABSTRACT
In Azospirillum brasilense ADP-ribosylation of dinitrogenase reductase (NifH) occurs in response to addition of ammonium to the extracellular medium and is mediated by dinitrogenase reductase ADP-ribosyltransferase (DraT) and reversed by dinitrogenase reductase glycohydrolase (DraG). The P(II) proteins GlnB and GlnZ have been implicated in regulation of DraT and DraG by an as yet unknown mechanism. Using pull-down experiments with His-tagged versions of DraT and DraG we have now shown that DraT binds to GlnB, but only to the deuridylylated form, and that DraG binds to both the uridylylated and deuridylylated forms of GlnZ. The demonstration of these specific protein complexes, together with our recent report of the ability of deuridylylated GlnZ to be sequestered to the cell membrane by the ammonia channel protein AmtB, offers new insights into the control of NifH ADP-ribosylation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Proteínas de Bactérias
/
Azospirillum brasilense
/
Membrana Celular
/
Processamento de Proteína Pós-Traducional
/
Proteínas PII Reguladoras de Nitrogênio
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article