Immunogenicity and immune modulation of osteogenic differentiated mesenchymal stem cells from Banna minipig inbred line.
Transplant Proc
; 38(7): 2267-9, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-16980061
ABSTRACT
Mesenchymal stem cells (MSCs) represent a good choice for cell transplantation due to their multilineage differentiation ability and low immunogenicity. Our previous in vitro studies indicated that undifferentiated swine MSCs show low immunogenicity suppressing the proliferative responses of human peripheral blood lymphocyte to several antigens. In this study, we investigated the immunogenicity and immune modulation ability of osteogenic differentiated MSCs. SLA class I (P1, P14) was detectable by reverse-transcriptase polymerase chain reaction on both differentiated and undifferentiated MSCs. SLA class II (SLA-DRA, SLA-DQA) was only detectable on differentiated MSCs mixed lymphocyte reaction assays demonstrated that both differentiated and undifferentiated MSCs failed to stimulate proliferative responses by human peripheral blood lymphocytes (hPBLs). Furthermore, as undifferentiated MSCs, osteogenic differentiated MSCs also suppressed hPBL proliferation to phytohemaglutinin.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
/
Células-Tronco
/
Porco Miniatura
/
Células da Medula Óssea
/
Mesoderma
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article