Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.
Nucleic Acids Res
; 34(18): e122, 2006.
Article
em En
| MEDLINE
| ID: mdl-17000637
ABSTRACT
The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern cloning techniques. Its application has, however, been hampered by incompatibility with proof-reading DNA polymerases. We have advanced the technique by identifying PfuCx as a compatible proof-reading DNA polymerase and by developing an improved vector design strategy. The original features of the technique, namely simplicity, speed, high efficiency and low cost are thus combined with high fidelity as well as a transparent, simple and flexible vector design. A comprehensive set of vectors has been constructed covering a wide range of different applications and their functionality has been confirmed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Uracila
/
Reação em Cadeia da Polimerase
/
Clonagem Molecular
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article