Monitoring interfacial bioelectrochemistry using a FRET switch.
J Phys Chem B
; 110(41): 20649-54, 2006 Oct 19.
Article
em En
| MEDLINE
| ID: mdl-17034255
ABSTRACT
Generation of functionally active biomolecular monolayers is important in both analytical science and biophysical analyses. Our ability to monitor the redox-active state of immobilized proteins or enzymes at a molecular level, from which stochastic and surface-induced variations would be apparent, is impeded by comparatively slow electron-transfer kinetics and associated signalnoise difficulties. We demonstrate herein that by covalently tethering an appropriate dye to the copper protein azurin a highly oxidation-state-sensitive FRET process can be established which enables redox switching to be optically monitored at protein levels down to the zeptomolar limit. The surface-potential-induced cycling of emission enables the redox potential of clusters of a few hundred molecules to be determined.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Azurina
/
Biofísica
/
Físico-Química
/
Cobre
/
Transferência Ressonante de Energia de Fluorescência
/
Eletroquímica
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article