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[Production of gastrodin through biotransformation of p-hydroxybenzaldehyde by cell suspension cultures of Datura tatula L].
Gong, Jia-Shun; Ma, Wei-Peng; Pu, Jun-Xue; Xu, Shu-Guan; Zheng, Shuang-Qing; Xiao, Chun-Jie.
Afiliação
  • Gong JS; Faculty of Food Science and Technology, Yunnan Agricultural University, Kunming, China. gong199@163.com
Sheng Wu Gong Cheng Xue Bao ; 22(5): 800-4, 2006 Sep.
Article em Zh | MEDLINE | ID: mdl-17037205
The conversion of exogenous p-hydroxybenzaldehyde to p-hydroxy-methyl-phenol-beta-D-glucoside (gastrodin) was studied by using cell suspension culture of Datura tatula L. The chemical structure of this synthesized gastrodin was identified based on the spectral analysis and chemical evidence. The conversion procedure of p-hydroxybenzaldehyde into gastrodin by D. tatula L. cell suspension cultures was established. The synthesized gastrodin (II) was isolated from the ferment liquor and identified by spectral analysis. At the same time, the p-hydroxybenzyl alcohol (I) converted through biotransformation of p-hydroxybenzaldehyde by cell suspension cultures of D. tatula L. was also isolated and identified. The efficiency of glucosylation of p-hydroxybenzaldehyde was remarkably enhanced by adding salicylic acid (0.1 mg/L) and keeping the lower pressure (0.001MPa) in 25L airlift loop bioreactor. The biotransformation of exogenous p-hydroxybenzaldehyde to gastrodin by cell suspension culture of D. tatula L. is a promising approach.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Benzaldeídos / Datura / Glucosídeos Idioma: Zh Ano de publicação: 2006 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Benzaldeídos / Datura / Glucosídeos Idioma: Zh Ano de publicação: 2006 Tipo de documento: Article