Replication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand.
EMBO J
; 25(22): 5358-71, 2006 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-17066082
ABSTRACT
Using two-dimensional agarose gel electrophoresis, we show that mitochondrial DNA (mtDNA) replication of birds and mammals frequently entails ribonucleotide incorporation throughout the lagging strand (RITOLS). Based on a combination of two-dimensional agarose gel electrophoretic analysis and mapping of 5' ends of DNA, initiation of RITOLS replication occurs in the major non-coding region of vertebrate mtDNA and is effectively unidirectional. In some cases, conversion of nascent RNA strands to DNA starts at defined loci, the most prominent of which maps, in mammalian mtDNA, in the vicinity of the site known as the light-strand origin.
Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ribonucleotídeos
/
DNA Mitocondrial
/
Replicação do DNA
/
Conformação de Ácido Nucleico
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article