A rapid liquid chromatography-tandem mass spectrometry method for quantification of a biologically active molecule camboginol in the extract of Garcinia cambogia.
Biomed Chromatogr
; 21(1): 55-66, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17080506
ABSTRACT
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of camboginol in the extract of fruit rinds of Garcinia cambogia has been developed. Separation was achieved isocratically on an RP C(18) column using a solvent system consisting of a mixture of acetonitrile-water (91) and methanol-acetic acid (99.50.5) in the ratio of 3070 as mobile phase at a flow rate of 0.4 mL/min. A multiple reaction monitoring (MRM) method was developed for quantification of camboginol in the fruit rinds extract of G. cambogia using MRM transitions of m/z 601.4 --> m/z 176.7 and m/z 601.4 --> m/z 448.9, respectively. The calibration curve based on peak area against concentration was linear up to 50 ng/mL with a detection limit of 0.5 ng/mL. The method showed satisfactory reproducibility with a coefficient of variation of less than 6%. The method was successfully applied for quantification of camboginol in different Garcinia extracts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Terpenos
/
Garcinia cambogia
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article