[Association between calcium-sensing receptor protein expression and rat cardiomyocyte apoptosis].
Zhonghua Xin Xue Guan Bing Za Zhi
; 34(8): 739-43, 2006 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-17081403
ABSTRACT
OBJECTIVE:
To investigate the relationship between calcium-sensing receptor protein (CaSR) expression and rat cardiomyocyte apoptosis and related signal transduction pathways.METHODS:
The CaSR, BCl2, Caspase3 protein and ERK1/2 phosphorylation or non-phosphorylation were detected by Western blot. Cardiomyocyte apoptosis was detected by flow cytometry and immunofluorescence.RESULTS:
CaSR protein was detected in rat cardiac tissue and CaSR activator gadolinium (GdCl3) induced cardiomyocyte apoptosis and increased ERK1/2 phosphorylation and expression of BCl2 and activated Caspase3. The selective mitogen-activated protein kinase (MAPK) inhibitor PD98059 abolished gadolinium -induced ERK1/2 activation and BCl2 expression, further increased the activation of Caspase3 and cardiomyocyte apoptosis.CONCLUSION:
Our results demonstrate the CaSR existence in cardiomyocytes and CaSR activation by gadolinium can induce myocyte apoptosis by activating Caspase3 and tyrosine protein kinase pathway.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Miócitos Cardíacos
/
Receptores de Detecção de Cálcio
/
Miocárdio
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2006
Tipo de documento:
Article