Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates.
Virology
; 360(1): 36-49, 2007 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-17098273
ABSTRACT
Although vesicular stomatitis virus (VSV) neurovirulence and pathogenicity in rodents have been well studied, little is known about VSV pathogenicity in non-human primates. To address this question, we measured VSV viremia, shedding, and neurovirulence in macaques. Following intranasal inoculation, macaques shed minimal recombinant VSV (rVSV) in nasal washes for 1 day post-inoculation; viremia was not detected. Following intranasal inoculation of macaques, wild type (wt) VSV, rVSV, and two rVSV-HIV vectors showed no evidence of spread to CNS tissues. However, macaques inoculated intrathalamically with wt VSV developed severe neurological disease. One of four macaques receiving rVSV developed clinical and histological signs similar to the wt group, while the remaining three macaques in this group and all of the macaques in the rVSV-HIV vector groups showed no clinical signs of disease and reduced severity of histopathology compared to the wt group. The implications of these findings for rVSV vaccine development are discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças do Sistema Nervoso Central
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Infecções por Rhabdoviridae
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Vírus da Estomatite Vesicular Indiana
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Vetores Genéticos
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Doenças dos Macacos
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article