PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.
J Clin Microbiol
; 45(2): 564-7, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17121998
ABSTRACT
We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Reação em Cadeia da Polimerase
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Fixação de Tecidos
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Inclusão em Parafina
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Naegleria fowleri
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Meningoencefalite
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article