Equal rates of repair of DNA photoproducts in transcribed and non-transcribed strands in Sulfolobus solfataricus.
Mol Microbiol
; 63(2): 521-9, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17163966
The nucleotide excision repair (NER) pathway removes bulky lesions such as photoproducts from DNA. In both bacteria and eukarya, lesions located in transcribed strands are repaired significantly faster than those located in non-transcribed strands due to damage signalling by stalled RNA polymerase molecules: a phenomenon known as transcription-coupled repair (TCR). TCR requires a mechanism for coupling the detection of stalled RNA polymerase molecules to the NER pathway, provided in bacteria by the Mfd protein. In the third domain of life, archaea, the pathway of NER is not well defined, there are no Mfd homologues and the existence of TCR has not been investigated. In this report we looked at rates of removal of photoproducts in three different operons of the crenarchaeon Sulfolobus solfataricus following UV irradiation. We found no evidence for significantly faster repair in the transcribed strands of these three operons. The rate of global genome repair in S. solfataricus is relatively rapid, and this may obviate the requirement for a specialized TCR pathway. Significantly faster repair kinetics were observed in the presence of visible light, consistent with the presence of a gene for photolyase in the genome of S. solfataricus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Arqueal
/
Sulfolobus solfataricus
/
Reparo do DNA
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article