[Separation of sitafloxacin epimers by capillary electrophoresis].
Se Pu
; 24(5): 513-5, 2006 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-17165551
ABSTRACT
Sitafloxacin epimers were separated by capillary zone electrophoresis using gamma-cyclodextrin (gamma-CD) and D-phenylalanine (D-Phe) as chiral selector. The effects of the concentrations of gamma-CD, D-Phe, Cu2+ and pH of buffer were investigated. An uncoated fused-silica capillary of 50 microm i.d. and 60 cm (effective length 52.5 cm) was used. The capillary temperature was maintained at 25 degrees C. Samples were injected under a pressure of 7 kPa for 5 s and separated at 15 kV. A baseline separation of sitafloxacin epimers was achieved with a background electrolyte of 10 mmol/L KH2PO4-K2HPO4(pH 4.5), 10 mmol/L CuSO4, 20 mmol/L gamma-CD and 10 mmol/L D-Phe. The linear range for sitafloxacin was 32 -400 mg/L (0.996). The relative standard deviations (RSDs) of migration time and peak area were less than 1.9% and 3.8% respectively. This method can be applied in qualitative and quantitative analysis for sitafloxacin epimers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fluoroquinolonas
Tipo de estudo:
Qualitative_research
Idioma:
Zh
Ano de publicação:
2006
Tipo de documento:
Article